Supplementary MaterialsS1 Table: Zebrafish transgenic lines and mutant used in this study. sheet time lapses at 5 min time resolution. Data were binned as developmental stage +/? 3 h. = 197 cells from 20 embryos (24 hpf = 20; 30 hpf = 56; 36 hpf = 57; 42 hpf = 53; 48 hpf = 11). (C) Retinal neurogenesis. Average number of neuronal subtypes, as analyzed by FACS from pooled dissected Tg(SoFa) retinal samples. = 20 retinas/stage. Data were normalized to wild-type background fluorescence. (D) Cell density was calculated by dividing the number of cells by total tissue volume. = 10 samples/stage. (Underlying data can be found at DOI: 10.5281/zenodo.1316912; for panels B and D at /Matejcic-et-al_2018/Data/F1_2_3D_S12BD34.csv, panel C at S2C.xlsx.). Ath5, atonal homolog 5; FACS, fluorescence-activated cell sorting; hpf, hours post fertilization.(TIFF) pbio.2006018.s006.tiff (610K) GUID:?22879D94-BFC0-4746-9D6F-41F9A8DF64BF S3 Fig: Mitotic cells at the apical surface of the retinal PSE. (A) Left: Schematic representation of PSE tissue architecture, with apical mitoses, migrating Aciclovir (Acyclovir) nuclei (arrows), and the mitotic frustum. The mitotic frustum is depicted as a conical unit below the rounded mitotic cell. We assume that all interphase nuclei in a single mitotic frustum (gray ellipses) undergo mitosis at the same position at the apical surface (gray). Middle: Schematic top view onto the apical surface cross-section (gray plane) marked in the left schematic. Interphase cells apical attachments are not shown. Right: Apical surface of the retinal PSE at 35 hpf, with cross-sections of mitotic and interphase cells. Cell membranes are labeled with Tg(actb1:HRAS-EGFP). Frame from Video 2. M: mitotic cells. Scale bar: 10 m. (B) Fraction of the apical tissue surface area occupied by mitotic cells; 10 samples/stage. Related to Fig 3G. (Underlying data can be found at DOI: 10.5281/zenodo.1316912; /Matejcic-et-al_2018/Data/F1_2_3D_S12BD34.csv.). hpf, hours post fertilization; PSE, pseudostratified epithelium.(TIFF) pbio.2006018.s007.tiff (787K) GUID:?BBFB14D0-AA62-476D-84D2-31BAE381F5A8 S4 Fig: Simplified description of zebrafish retina growth between 20 hpf and 48 hpf. (A) Schematic of division and differentiation rules considered in the simplified description of retina growth. For simplicity, we consider 2 cell populationsprogenitors (white) and neurons, or committed precursors (gray). Progenitors divide with a constant rate = 1. (B) Schematic of cell and tissue shape geometry. Cells are represented by truncated cones with apical and basal line tensions and = 5) and in hdac1?/? tissue treated with 150 M Rockout (= 6). Rockout treatment abolishes the basolateral actin accumulation in Aciclovir (Acyclovir) hdac1?/? and restores the basal-to-lateral actin ratio to control values. Mean SD. Mann-Whitney test, control versus hdac1?/? salivary gland [1] or the vertebrate retina [2], shape characteristics are established early in development and need to be retained throughout growth. This Rabbit Polyclonal to RAB41 necessitates an isotropic rescaling of the initial tissue shape (Fig 1A). How such uniform, isotropic rescaling is achieved through cell and tissue level processes, however, is not yet well explored. Open in a separate window Fig 1 A 3D tissue-wide analysis allows cell-level investigation of tissue Aciclovir (Acyclovir) shape maintenance during vertebrate retinal PSE growth.(A) Schematic of vertebrate retinal development. After the optic vesicle forms the optic cup, cells in the retinal PSE proliferate as the tissue maintains its shape (20C42 hpf) to ultimately give rise to the laminated neuronal retina. (A) The developing vertebrate retina is a PSE. Left: Optical slice through the retinal PSE at approximately 30 hpf, with a single cell outlined (dashed white line). Apical and basal surfaces of the tissue are outlined (dashed white lines). Cell membranes are labeled by Tg(actb1::HRAS-EGFP). Scale bar: 20 m. Right: Schematic of.