Compressive sensing, also known as compressive decoding or compressive sampling, is a signal processing technique which aims to recover an original sparse signal based on a subsampling of measurements in which the sampling rate is below the traditional rate defined by the Nyquist-Shannon sampling theorem. computation, the acquired images can achieve a wide field of view (FOV) of ~113?mm2 as well as a cellular resolution of ~3?m, which enables various forms of follow-up image-based cell analysis. We performed 12?hours time-lapse study on paclitaxel-treated MCF-7 and HEK293T cell lines using w-SCOPE. The analytic results, such as the 8-Hydroxyguanine calculated viability and therapeutic window, from our device were validated by standard cell detection assays and imaging-based cytometer. In addition to those end-point detection methods, w-SCOPE further uncovered the time course of the cells response to the drug treatment over the whole period of drug exposure. Light microscopy is a widely used technique that brings insight into modern life science research by enabling visualization of microscopic phenomena. Numerous light microscopy techniques based on different principles have been invented in the past century1,2,3,4,5,6. In spite of the various modalities, microscopes in the common sense generally involve fairly complicated settings with large form factors and high upkeep. Therefore, for a long time, access to microscopes, especially fluorescent microscopes, has been limited to highly specialized sites, such as hospitals and research laboratories. Recently, several types of portable, cost-effective light microscopes have emerged7,8,9,10,11,12,13. Imaging with these portable microscopes is accomplished by using small optics and electronics7,8,10,11. In some modalities9,12,13, even the lens elements, generally the most essential components for imaging, are eliminated to drastically reduce the size 8-Hydroxyguanine of the device and to circumvent the need to find a appropriate balance between field-of-view and resolution14. To produce an image with both high resolution and large FOV, a series of post-processing strategies, such as pixel super-resolution12,15, in-line digital holography reconstruction15,16 and compressive sensing8,9, are used to compensate for the unsatisfactory quality captured from the limited optical power. These compact and lightweight microscope products for bright-field and fluorescent imaging are desired for use in resource-limited environments17. Most of the aforementioned compact microscope products are optimized for stained deceased cell analysis. These devices are exempt from the requirement of a dedicated environment with stable humility, temp and CO2 concentration, which is necessary for long-term live cell observation. However, observing changes in live cells over a period of time, known as time-lapse or longitudinal microscopy, is essential to a variety of cell biology study areas. Examples of its uses include aiding in drug testing18, visualizing cell apoptotic processes19, analyzing cell division phenotypes20 and investigating gene function by RNA interference21. Currently, the dominating method to create a stable and appropriate environment for cellular growth while concurrently observing the cells is definitely to build a customized incubator on an existing microscope due to the infeasibility of Rabbit Polyclonal to HBP1 bringing the heavy microscope into a CO2 8-Hydroxyguanine incubator. Aside from the cumbersome form element, the conventional incubator-on-microscope modality requires considerable expense due to the necessity of the unique incubator. In the mean time, time-lapse imaging of cell tradition has an intrinsic need for wide FOV, to track a larger human population of cells for better statistical analysis over extended periods of time. In contrast, the conventional microscopes frequently used for housing the incubator and accommodating the cell tradition typically has a minimum magnifying power of two, which causes a limited FOV no larger than 40 mm2 in the acquired digital images. Image stitching techniques are usually employed in this case, to stitch multiple small frames into a solitary big one, to accomplish sufficiently large FOV. For this method, any failed image necessitates repetition of the entire acquisition, requiring 100% reliability for each frame captured during the observation period22. Moreover, the system needs to become equipped with additional high precision motorized parts23, adding to the difficulty of the system. Recently, several compact, lens-based and lens-free imaging products characterized by low cost and modestly large field-of-view have been reported for dynamic observation of living cells23,24,25,26,27,28,29,30,31. In lens-based modalities, the mini-microscope is definitely portable and allows easy integration with a wide 8-Hydroxyguanine variety of pre-existing platforms, such as petri dishes, cell tradition plates, and microfluidic bioreactors, for chronologically monitoring the cell dynamics30,31. In lens-free modalities, to harvest adequate resolution in the recorded raw images24,28, microfluidic chambers were specially designed to tradition the cells and more importantly, place them close to the image sensor surface. As a result, the FOV accomplished in lens-free establishing is essentially fixed and can become as large as the active area of the sensor. For further improving the native resolution limited by the pixel size of the image sensor, multiple shift-correlated images of the cells could also be produced in lens-free modalities, by exactly scanning the illumination resource25,27,29 or taking advantage of the inherent motion of the microorganisms26..